سال انتشار: ۱۳۸۸

محل انتشار: اولین کنفرانس بین المللی تصفیه فاضلاب و بازیافت آب، فناوری ها و یافته های نو

تعداد صفحات: ۶

نویسنده(ها):

S Abdollahpour – National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.BOX 14155-6343, Tehran, Iran – Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
M Mollaei – National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.BOX 14155-6343, Tehran, Iran
S Atashgahi – National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O.BOX 14155-6343, Tehran, Iran
A Sahebghadam Lotfi – Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran

چکیده:

In this investigation, single and hybrid immobilization techniques for whole cells of Pseudomonas sp. SA01 were employed to enhance phenol degradation rate. The single immobilization was achieved using alginate (2-4%) and pectin (3-5%) together with the combined immobilization systems which consisted of alginate-chitosan-alginate (ACA), polyvinyl alcohol (PVA)-alginate capsules. The freely suspended cellsof Pseudomonas sp. SA01 was able to degrade phenol in concentrations up to 1000 mg/L 84 h. The complete degradation of the same concentration of phenol by cells entrapped in alginate (3%), in hybrid immobilization system alginate-chitosan-alginate (ACA), and in PVA-alginate encapsulated cells were achieved as low as 30 h. However, the cells immobilized in pectin (5 %) could degrade the same concentration of phenol at 35 and 40 h respectively. The cell loading capacity is increased in PVA-alginate hybrid capsules owing to the reduction of cell leakage from the beads, resulted in higher activity of immobilized cells compared with the single immobilization method.