سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۴
Navid Dinparast Djadid – Malaria Research Group (MRG), Biotechnology Dept., Pasteur Institute of Iran
Flora Forouzesh – Malaria Research Group (MRG), Biotechnology Dept., Pasteur Institute of Iran
Sedigheh Zakeri –
An. sacharovi and An. maculipennis are major malaria vectors in North of Iran. During the last 50 years, most of the main vector species have been under pressure of all four generations of insecticide. In this study, we carried out the susceptibility test on field-collected specimens of An. sacharovi and An. Maculipennis, followed by detection of knockdown resistance (kdr) mutation in segment 6, domain II of the voltage-gated sodium channel gene (VGSC), as an important resistance mechanism against pyrethroids. The total size of amplified fragment were 225bp in An. sacharovi and 216bp in An. maculipennis. These fragments were purified and sequenced. An point mutation involving a single A to G in the DDT-resistant strain was detected. This mutation appears to be homologous with those detected in An. sacharovi in Turkey. Comparison of VGSC gene based on nucleotide sequences indicated 93% similarity between An. sacharovi and An. maculipennis, but amino acid sequence did not change. Further analysis on exonI and exonII revealed that all nucleotide changes are similar in different specimens of An. sacharovi, expect in position 42 of 63Pars strain, which resulted in polymorphism within this species but with no amino acid change in different examined individuals.