سال انتشار: ۱۳۸۷

محل انتشار: دومین کنگره بین المللی علوم و فناوری نانو

تعداد صفحات: ۲

نویسنده(ها):

M Majdinasab – Department of Food Science and Technology, University of Shiraz, P. O. Box71345-1789, Shiraz, Iran
M Aminlari –
M. H. Sheikhi, –
M Niakousari –

چکیده:

Salmonella is an important pathogen to the food industry and has been frequently identified as the etiological agent of foodborne outbreaks [1]. Salmonella is responsible for more than 1 million outbreaks, resulting in 500+ deaths every year in the United States, and is the leading causative agent among nine foodborne bacterial pathogens resulting in 39% of 15,600 laboratory-diagnosed infections in 2003. A majority of cases of humansalmonellosis are due to the consumption of contaminated egg, poultry, pork, beef, and milk products. Conventional culture methods used for the detection of Salmonella include non-selective preenrichment, followed by enrichment and plating on selective and differential agars. Suspect colonies are confirmed biochemically and serologically, possibly taking up to 7 days to complete the entire procedure [2]. More recently, a number of rapid methods for detection of Salmonella in foods have been developed. Goldnanoparticles have been used in biotechnology over the last 4 decades as immunocytochemical probes as well as biological tags [3]. During the last decade, however, there has been an increasing interest in using nanoparticles in DNA detection [4]. Recent advances in functionalizing particles with oligonucleotides and tailoring their surface properties have paved the way for the development of a series of new and practical biodetection systems [5].This paper reports a new ultra-sensitive nanosensor based on color shift, where selective recognition isaccomplished using gold nanoparticles functionalized with ssDNA probes that recognize and bind to target complementary DNA of Salmonella inv A gene