سال انتشار: ۱۳۹۰

محل انتشار: پنجمین کنفرانس بین المللی پیشرفتهای علوم و تکنولوژی

تعداد صفحات: ۶

نویسنده(ها):

z Rastegar – MSc Student of Iran University of Science and Technology, Tehran, Iran
M Haghpanahi – Professor assistant of Iran University of Science and Technology, Tehran, Iran
M.H Korayem – Professor of Iran University of Science and Technology, Tehran, Iran
M Geramizadeh –

چکیده:

The realization of promising potential of stem cells for engineering bone, cartilage, muscle and other connective tissues requires a proper characterization of their unique biological, biochemical, proteomic, and biomechanical properties that are yet to be fully elucidated. The mechanical properties such as elasticity, membrane tension, cell shape, and adhesion strength may play an important role in the cell fate and differentiation. The mechanical properties of biological cells have been studied with different techniques; the most popular ones are optical tweezers, magnetic beads, and micropipette aspiration. However, those methods cannot compete with the precision that can be attained with atomic force microscopy (AFM) method. AFM has been widely used in the study of micro and nanostructures including living cells. As the interpretation of atomic force microscopy-based indentation tests is highly dependent on the use of an appropriate theoretical model of the testing configuration, in this paper various contact models were presented to predict the mechanical behavior of individual stem cells. Due to special conditions assumed, the most proper one was chosen. A comparison study with finite element simulations (FEM) of spherical tip indentation demonstrates the effectiveness of our computational model to predict the cell deformation during indentation tasks.