سال انتشار: ۱۳۸۸

محل انتشار: اولین کنفرانس بین المللی تصفیه فاضلاب و بازیافت آب، فناوری ها و یافته های نو

تعداد صفحات: ۷

نویسنده(ها):

S Motamed – Department of Chemical Engineering, Sharif University of Technology, Tehran, Iran
F Ghaemmaghami – Department of Chemical Engineering, Sharif University of Technology, Tehran, Iran
I Alemzadeh – Department of Chemical Engineering, Sharif University of Technology, Tehran, Iran

چکیده:

Phenolic compounds are one of the major classes of organic pollutants generated through various industrial activities. Among the novel treatment options, an enzymatic process using a peroxidase, such as radish peroxidase, and hydrogen peroxide has proven very effective in removing a variety of phenolic pollutants from aqueous solutions with high efficiency and selectivity. A plant peroxidase extracted from White Radishwaspartially purified by extraction using a polyethylene glycol: ammonium sulphate aqueous two-phase system, followed by gel filtration on a Sephadex G-200 column. Variation of phase composition and NaCl concentration resulted in the desired reduction in volume of the extract and selective partitioning of the enzyme. PEG:ammonium sulphate:NaCl (27:6:2%, w:v) system gave a partition coefficient of 0.18, purification factor of 3.57, volume reduction of 58.75% and bottom phase recovery of 71%. The enzyme was further concentrated by anti-dialysis and was finally purified about 36-fold by gel filtration on a Sephadex G-200 column, with a recovery of 61%. The enzyme was stable at 65°C with an optimum activity at 55°C. The enzyme was stable at pH range 4 – 8 and had an optimum pH at 4.0.