سال انتشار: ۱۳۹۰

محل انتشار: دومین همایش ملی مدیریت کنترل آفات

تعداد صفحات: ۱

نویسنده(ها):

Fariba Mehrkhou – Department of plant protection, Faculty of Agriculture, Urmia University. Urmia, Iran
Ali Asghar Talebi – Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O.Box 14115-336, Tehran, Iran..
Saeed Moharramipour – Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, P.O.Box 14115-336, Tehran, Iran..
Vahid Hosseininaveh – Department of Plant Protection, College of Agricultural Sciences and Natural Resources University of Tehran, Karaj, Iran

چکیده:

The beet armyworm, Spodoptera exigua (Lep.: Noctuidae) is an important and polyphagous pest inIran. Effect of nine soybean cultivars (033, Hill, L17, BP and Zane) on proteolytic activities of digestiveenzymes of the beet armyworm was determined at 25 ± ۱°C, 60 ± ۵% RH and a photoperiod of 16:8(L:D) hours. For sample preparation, the midgut of about 2-day-old fifth instar larvae of S. exigua, wereused. Midgut extracts from larvae showed an optimum activity for proteolysis against azocasein atalkaline pH (10). Casein zymogram analysis revealed 4-7 proteinase bands in the larval midgut extractand strongly showed the presence of trypsin-like serine proteinases. Although the highest total proteolyticactivity was on larvae fed on Zane and Bp varieties, the lowest tryptic activity was observed also on Zaneand BP, which could be due to the presence of serine protease inhibitors (TLCK) in these two varieties,resulting in hyper production of chymotrypsin in response to the inhibition of the serine protease.