سال انتشار: ۱۳۹۱

محل انتشار: اولین کنفرانس بین المللی مواد پیشرفته

تعداد صفحات: ۳

نویسنده(ها):

Nahid Hossein Fakhrabadi – Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Ali Asghar Rohani – Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Afshin Farahbakhsh – Department of Chemical Engineering, Quchan Branch, Islamic Azad University, Quchan, Iran
Saeed Sohrabi –

چکیده:

Using glucose biosensor is important in detection various amounts of glucose. In this paper, a biosensor electrode called (Aunano/CPE) electrode was prepared which is a modified carbon fermentation electrode with colloidal gold nanoparticles composed of carbon graphite powder, paraffin oil and colloidal gold nanoparticles (24nm dia.) and put in contact with glucose oxidase enzyme in the presence of phosphate buffer by a semi-permeable membrane. Ag/AgCl reference electrode and (Aunano/CPE) electrode were used for performing biosensor amperometric and glucose was detected in a definite concentration range by applying 0.7V constant potential to the electrodes. Experiment design was performed by Taguchi method for both glucose concentration and pH factors on three levels for which the optimum values were obtained as 1mM and 6, respectively. The biosensor remained stable for 6 days based on the activity of glucose oxidase enzyme in optimum conditions