سال انتشار: ۱۳۹۱

محل انتشار: چهاردهمین کنگره ملی مهندسی شیمی ایران

تعداد صفحات: ۷

نویسنده(ها):

Eiman Rahnema Falavarjani – Sharif University of Technologh
Mohammad Jafar Abde khodaie –
Ali Nematollah zade –
Mahmood Rahimi Zarchi –

چکیده:

In the past few years, there has been an increasing interest in the encapsulation of mammalian cells. The use of sodium alginate(SA) as polymer base in the encapsulation of sensitive human living cells needs the control of conditions of system to keep cells alive. In this study, the cells interaction with alginate during gelling process and after synthesizing of Calcium Alginate (Ca-Al) is evaluated by tracing of pH changes. Also the effects of different buffer base solvents were observed in control of pH value during gelling process. However finally we figured out buffer singly is not workable to regulate biological system. As matter of fact, the type of buffer, concentration of solutions and the refreshing time of solution in system are essential parameters on regulation of pH value. In the other word, we present here in vitro biological system in which cell immobilization can be achieved in optimal way